Workflows

In rd10 or other RP lines imaged with the Envisu, you will see a progressive thinning of the outer retina while the inner retinal layers (like the ganglion cell layer and inner plexiform layer) remain relatively preserved in the early-to-mid stages of the disease.​

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SD-OCT imaging of wild-type mice demonstrated visibility of the inner segment/outer segment (IS/OS) junction, external limiting membrane (ELM), outer nuclear layer (ONL), and outer plexiform layer (OPL). At P14, most rd10 mice exhibited normal SD-OCT profiles, but some displayed changes in the IS/OS junction. At the same time point, rd1 mice had severe outer retinal degeneration. In rd10 mice, imaging revealed loss of the IS/OS junction by P18, hyperreflective changes in the ONL at P20, hyperreflective vitreous opacities, and shallow separation of the neural retina from the RPE.

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Pennesi, Mark & Michaels, Keith & Magee, Sienna & Maricle, Anastasiya & Davin, Sean & Garg, Anupam & Gale, Michael & Tu, Daniel & Wen, Yuquan & Erker, Laura & Francis, Peter. (2012). Long-Term Characterization of Retinal Degeneration inrd1andrd10Mice Using Spectral Domain Optical Coherence Tomography. Investigative Opthalmology & Visual Science. 53. 4644. 10.1167/iovs.12-9611. ​

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